A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Eperisone Hydrochloride (EPE) and Diclofenac Sodium (DICLO) by employing first order derivative zero crossing method in Distilled water. The first order derivative absorption at 261 nm (zero cross point of Eperisone Hydrochloride) was used for quantification of Diclofenac Sodium and 249 nm (zero cross point of Diclofenac Sodium) for quantification of Eperisone Hydrochloride. The linearity was established over the concentration range of 4-20 µg/ml and 4-20 µg/ml for Eperisone Hydrochloride and Diclofenac Sodium with correlation coefficient r2 0.9987 and 0.9982, respectively. The mean % recoveries were found to be in the range of 99.97%–101.16% and 98.66%–100.13% for Eperisone Hydrochloride and Diclofenac Sodium, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Eperisone Hydrochloride and Diclofenac Sodium in Pharmaceutical Dosage Form.
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